PDE1C Kit ELISA

N° du produit ABIN5674918

Détail du produit PDE1C Kit ELISA

Antigène: PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 0055 ng/mL

Ingrédients: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Matériel non inclus: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))

Autre désignation: Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) (PDE1C Produits)

Synonymes: CG14940 Kit ELISA, CG14942 Kit ELISA, CG14943 Kit ELISA, CG14944 Kit ELISA, CG31757 Kit ELISA, CG31758 Kit ELISA, CG42325 Kit ELISA, CG44007 Kit ELISA, Dmel\\CG44007 Kit ELISA, Dmel_CG14940 Kit ELISA, Dmel_CG14943 Kit ELISA, Dmel_CG14944 Kit ELISA, Dmel_CG31757 Kit ELISA, Dmel_CG31758 Kit ELISA, Dmel_CG42325 Kit ELISA, PDE1C Kit ELISA, PDE1c Kit ELISA, GB13690 Kit ELISA, Hcam3 Kit ELISA, Phosphodiesterase 1c Kit ELISA, phosphodiesterase 1C Kit ELISA, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A Kit ELISA, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C Kit ELISA, phosphodiesterase 1C, calmodulin-dependent b Kit ELISA, Pde1c Kit ELISA, PDE1C Kit ELISA, LOC724389 Kit ELISA, LOC100019026 Kit ELISA, pde1cb Kit ELISA

Sujet: Alternative name: Hcam3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C

ID gène: 5137

UniProt: Q14123

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma