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PDE1C Kit ELISA

PDE1C Reactivité: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
N° du produit ABIN5674919
  • Antigène Voir toutes PDE1C Kits ELISA
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    Reactivité
    • 2
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.156 ng/mL - 10 ng/mL
    Seuil minimal de détection
    0.156 ng/mL
    Application
    ELISA
    Type d'échantillon
    Tissue Homogenate
    Analytical Method
    Quantitative
    Sensibilité
    0042 ng/mL
    Ingrédients
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    Matériel non inclus
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Durée du test
    1 - 4.5 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    12 months
  • Antigène Voir toutes PDE1C Kits ELISA
    PDE1C (phosphodiesterase 1C, Calmodulin-Dependent 70kDa (PDE1C))
    Autre désignation
    Phosphodiesterase 1C, Calmodulin Dependent (PDE1C) (PDE1C Produits)
    Synonymes
    CG14940 Kit ELISA, CG14942 Kit ELISA, CG14943 Kit ELISA, CG14944 Kit ELISA, CG31757 Kit ELISA, CG31758 Kit ELISA, CG42325 Kit ELISA, CG44007 Kit ELISA, Dmel\\CG44007 Kit ELISA, Dmel_CG14940 Kit ELISA, Dmel_CG14943 Kit ELISA, Dmel_CG14944 Kit ELISA, Dmel_CG31757 Kit ELISA, Dmel_CG31758 Kit ELISA, Dmel_CG42325 Kit ELISA, PDE1C Kit ELISA, PDE1c Kit ELISA, GB13690 Kit ELISA, Hcam3 Kit ELISA, Phosphodiesterase 1c Kit ELISA, phosphodiesterase 1C Kit ELISA, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A Kit ELISA, calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C Kit ELISA, phosphodiesterase 1C, calmodulin-dependent b Kit ELISA, Pde1c Kit ELISA, PDE1C Kit ELISA, LOC724389 Kit ELISA, LOC100019026 Kit ELISA, pde1cb Kit ELISA
    Sujet
    Alternative name: Hcam3, Cam-PDE 1C, Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1C
    ID gène
    81742
    UniProt
    Q63421
    Pathways
    EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Negative Regulation of Hormone Secretion, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma
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