beta Arrestin 1 Kit ELISA

N° du produit ABIN5690830

Détail du produit beta Arrestin 1 Kit ELISA

Antigène: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human beta-Arrestin-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human beta-Arrestin-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Autre désignation: beta-Arrestin-1 (ARRB1 Produits)

Synonymes: arrb1 Kit ELISA, MGC83787 Kit ELISA, arb1 Kit ELISA, arr1 Kit ELISA, barr1 Kit ELISA, betaarr1 Kit ELISA, 1200006I17Rik Kit ELISA, AW208571 Kit ELISA, G430100A01Rik Kit ELISA, ARB1 Kit ELISA, ARR1 Kit ELISA, BARRES Kit ELISA, arrestin beta 1 Kit ELISA, arrestin, beta 1 Kit ELISA, arrestin beta 1 L homeolog Kit ELISA, beta-arrestin 1 Kit ELISA, putative beta-arrestin 1 Kit ELISA, ARRB1 Kit ELISA, arrb1 Kit ELISA, arrb1.L Kit ELISA, CpipJ_CPIJ016988 Kit ELISA, CpipJ_CPIJ017114 Kit ELISA, Smp_122900 Kit ELISA, Smp_126080 Kit ELISA, Smp_176980 Kit ELISA, Smp_152290 Kit ELISA, Arrb1 Kit ELISA

ID gène: 408

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction