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IL-22 Kit ELISA

IL22 Reactivité: Mouton Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690981
  • Antigène Voir toutes IL-22 (IL22) Kits ELISA
    IL-22 (IL22) (Interleukin 22 (IL22))
    Reactivité
    • 11
    • 5
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouton
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Ovine (sheep) IL-22 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Ovine (sheep) IL-22.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes IL-22 (IL22) Kits ELISA
    IL-22 (IL22) (Interleukin 22 (IL22))
    Autre désignation
    IL-22 (IL22 Produits)
    Synonymes
    IL-21 Kit ELISA, IL-22 Kit ELISA, IL-D110 Kit ELISA, IL-TIF Kit ELISA, ILTIF Kit ELISA, TIFIL-23 Kit ELISA, TIFa Kit ELISA, zcyto18 Kit ELISA, IL-22a Kit ELISA, ILTIFa Kit ELISA, Iltif Kit ELISA, RGD1561292 Kit ELISA, interleukin-22 Kit ELISA, interleukin 22 Kit ELISA, IL22 Kit ELISA, il22 Kit ELISA, Il22 Kit ELISA
    ID gène
    100885770
    UniProt
    H2AKX1
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