TEK Kit ELISA

N° du produit ABIN5691020

Ce kit ELISA Poisson zèbre (Danio rerio) TEK est un kit ELISA Colorimetric conçu pour quantifier Poisson zèbre (Danio rerio) TEK.

Aperçu rapide pour TEK Kit ELISA (ABIN5691020)

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivité: Poisson zèbre (Danio rerio)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Détail du produit TEK Kit ELISA

Fonction: Zebrafish Tie-2 ELISA Kit.

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Zebrafish Tie-2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Autre désignation: Tie-2

ID gène: 30747

UniProt: O73791

Pathways: Signalisation RTK, Growth Factor Binding