PLGF Kit ELISA

N° du produit ABIN5692237

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris PLGF.

Aperçu rapide pour PLGF Kit ELISA (ABIN5692237)

Antigène: PLGF (PGF) (Placenta Growth Factor (PGF))

Épitope: AA 24-158

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit PLGF Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: Sandwich Mouse PLGF-2 / PGF ELISA Kit to quantitate Mouse Pgf in cell culture supernatants, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: A24-P158

Capture antibody and Detection antibody: monoclonal antibody from rat, biotinylated polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.1%, 6.9%, 4.3%
Inter-Assay Precision (CV%): 6.6%, 7.6%, 4.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Détail du antigène

Antigène: PLGF (PGF) (Placenta Growth Factor (PGF))

Autre désignation: Pgf

Sujet:

Background: Placental growth factor is a protein that in humans is encoded by the PGF gene. Placental growth factor (PGF) is a member of the VEGF (vascular endothelial growth factor) sub-family - a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. PGF is also expressed in many other tissues, including the villous trophoblast. PGF is ultimately associated with angiogenesis. Specifically, PGF plays a role in trophoblast growth and differentiation. Trophoblast cells, specifically extravillous trophoblast cells, are responsible for invading maternal arteries. Under normal physiologic conditions, PGF is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal skeletal muscle. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF-1, PlGF-2 and PlGF-3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described.

Gene Full Name: placental growth factor

ID gène: 18654

UniProt: Q544A5

Pathways: VEGFR1 Specific Signals