RAB8B Kit ELISA

N° du produit ABIN5706926

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain RAB8B.

Aperçu rapide pour RAB8B Kit ELISA (ABIN5706926)

Antigène: RAB8B (RAB8B, Member RAS Oncogene Family (RAB8B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156 ng/mL - 10 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit RAB8B Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Analytical Method: Quantitative

Sensibilité: 0.055 ng/mL

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Volume d'échantillon: 100 µL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: RAB8B (RAB8B, Member RAS Oncogene Family (RAB8B))

Sujet: Alternative name: RAB-8b Protein, Ras-Related Protein Rab-8B

Pathways: Positive Regulation of Peptide Hormone Secretion, Cell-Cell Junction Organization