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IL1A Kit ELISA

IL1A Reactivité: Humain Colorimetric Sandwich ELISA 0.5-300 pg/mL Cell Lysate, Tissue Lysate
N° du produit ABIN625018
  • Antigène Voir toutes IL1A Kits ELISA
    IL1A (Interleukin 1 alpha (IL1A))
    Reactivité
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.5-300 pg/mL
    Seuil minimal de détection
    0.5 pg/mL
    Application
    ELISA
    Fonction
    Human IL-1 alpha (IL-1 F1) ELISA Kit for cell and tissue lysate samples.
    Type d'échantillon
    Tissue Lysate, Cell Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human IL-1 alpha.
    Sensibilité
    0.5 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    • Cell lysate buffer
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes IL1A Kits ELISA
    IL1A (Interleukin 1 alpha (IL1A))
    Autre désignation
    IL-1 alpha (IL1A Produits)
    Synonymes
    IL-1A Kit ELISA, IL1 Kit ELISA, IL1-ALPHA Kit ELISA, IL1F1 Kit ELISA, IL-6 Kit ELISA, Il-1a Kit ELISA, IL-1 alpha Kit ELISA, IL-1alpha Kit ELISA, L1A Kit ELISA, interleukin 1 alpha Kit ELISA, interleukin 6 Kit ELISA, IL1A Kit ELISA, IL6 Kit ELISA, Il1a Kit ELISA
    Sujet
    Gene Names: IL1A IL1F1
    Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
    ID gène
    3552
    UniProt
    P01583
    Pathways
    Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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