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IL5RA Kit ELISA
IL5RA
Reactivité: Humain
Colorimetric
Sandwich ELISA
0.15-40 ng/mL
Cell Culture Supernatant, Plasma, Serum
Détail du produit IL5RA Kit ELISA
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Antigène
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IL5RA
(Interleukin 5 Receptor, alpha (IL5RA))
Reactivité
Toutes les réactivités sur IL5RA Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
0.15-40 ng/mL
Seuil minimal de détection
0.15 ng/mL
Application
ELISA
Fonction
Human IL-5 R alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensibilité
150 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IL5RA Kits ELISA
IL5RA
(Interleukin 5 Receptor, alpha (IL5RA))
Autre désignation
IL-5 R alpha (IL5RA Produits )
Synonymes
MGC86517 Kit ELISA, IL5RA Kit ELISA, il5r Kit ELISA, cdw125 Kit ELISA, hsil5r3 Kit ELISA, gpIL-5ra Kit ELISA, CD125 Kit ELISA, CDw125 Kit ELISA, HSIL5R3 Kit ELISA, IL5R Kit ELISA, Il5r Kit ELISA, interleukin 5 receptor subunit alpha L homeolog Kit ELISA, interleukin 5 receptor subunit alpha Kit ELISA, interleukin 5 receptor, alpha Kit ELISA, il5ra.L Kit ELISA, IL5RA Kit ELISA, il5ra Kit ELISA, Il5ra Kit ELISA
Sujet
Gene Names: IL5RA IL5R Protein names: Interleukin-5 receptor subunit alpha (IL-5 receptor subunit alpha) (IL-5R subunit alpha) (IL-5R-alpha) (IL-5RA) (CDw125) (CD antigen CD125)
ID gène
3568
UniProt
Q01344
Pathways
Signalistation JAK/STAT
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