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TYRO3 Kit ELISA

TYRO3 Reactivité: Souris Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625385
  • Antigène Voir toutes TYRO3 Kits ELISA
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Reactivité
    • 5
    • 3
    • 2
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.4-100 ng/mL
    Seuil minimal de détection
    0.4 ng/mL
    Application
    ELISA
    Fonction
    Mouse Dtk ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- ?, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 ?, IL-1?, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1?, MIP-1?, MIP-2, MIP-3?, MIP-3?, PF-4, P-Selectin, RANTES, SCF, SDF-1?, TARC, TCA-3, TECK, TIMP-1, TNF-?, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
    Sensibilité
    0.4 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes TYRO3 Kits ELISA
    TYRO3 (TYRO3 Protein Tyrosine Kinase (TYRO3))
    Autre désignation
    Dtk (TYRO3 Produits)
    Synonymes
    TYRO3 Kit ELISA, brt Kit ELISA, byk Kit ELISA, dtk Kit ELISA, rse Kit ELISA, sky Kit ELISA, tif Kit ELISA, Xksy Kit ELISA, MGC145734 Kit ELISA, DKFZp469I0432 Kit ELISA, LOC100231598 Kit ELISA, BYK Kit ELISA, Dtk Kit ELISA, RSE Kit ELISA, Sky Kit ELISA, Tif Kit ELISA, Brt Kit ELISA, REK Kit ELISA, ksy Kit ELISA, xksy Kit ELISA, AI323366 Kit ELISA, Etk-2 Kit ELISA, Rse Kit ELISA, TYRO3 protein tyrosine kinase Kit ELISA, TYRO3 protein tyrosine kinase L homeolog Kit ELISA, TYRO3 protein tyrosine kinase 3 Kit ELISA, TYRO3 Kit ELISA, tyro3 Kit ELISA, Tyro3 Kit ELISA, tyro3.L Kit ELISA
    Sujet
    Gene Names: Tyro3 Dtk Rse Tif
    Protein names: Tyrosine-protein kinase receptor TYRO3 (EC 2.7.10.1) (Etk2/tyro3) (TK19-2) (Tyrosine-protein kinase DTK) (Tyrosine-protein kinase RSE) (Tyrosine-protein kinase TIF)
    ID gène
    22174
    UniProt
    P55144
    Pathways
    Signalisation RTK
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