PCNA Kit IQ-ELISA

N° du produit ABIN6384893

Détail du produit PCNA Kit IQ-ELISA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) PCNA ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensibilité: 0.246 ng/mL

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• PCNA Microplate (Item A): 96 well PCR plate coated with anti-Human PCNA
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human PCNA
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for PCNA (Item F): 2 vials of a 4x concentrated solution of anti-Human PCNA affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA (PCNA Produits)

Synonymes: PCNAR Kit IQ-ELISA, Pcna/cyclin Kit IQ-ELISA, pcna Kit IQ-ELISA, AO090003000846 Kit IQ-ELISA, 53/13 Kit IQ-ELISA, CG9193 Kit IQ-ELISA, DmPCNA Kit IQ-ELISA, Dmel\\CG9193 Kit IQ-ELISA, MUS209 Kit IQ-ELISA, Mus209 Kit IQ-ELISA, PCNA Kit IQ-ELISA, PCNA/mus209 Kit IQ-ELISA, Pcna Kit IQ-ELISA, dPCNA Kit IQ-ELISA, l(2)02448 Kit IQ-ELISA, l(2)56F Kit IQ-ELISA, l(2)56Fa Kit IQ-ELISA, l(2)s1534 Kit IQ-ELISA, cb16 Kit IQ-ELISA, fb36g03 Kit IQ-ELISA, wu:fa28e03 Kit IQ-ELISA, wu:fb36g03 Kit IQ-ELISA, pcna-A Kit IQ-ELISA, proliferating cell nuclear antigen Kit IQ-ELISA, DNA polymerase III sliding clamp Kit IQ-ELISA, Proliferating cell nuclear antigen Kit IQ-ELISA, Proliferating cell nuclear antigen-like Kit IQ-ELISA, proliferating cell nuclear antigen L homeolog Kit IQ-ELISA, proliferating cell nuclear antigen 1 Kit IQ-ELISA, PCNA Kit IQ-ELISA, Pcna Kit IQ-ELISA, pcna Kit IQ-ELISA, MMP_RS08810 Kit IQ-ELISA, ANI_1_512014 Kit IQ-ELISA, AOR_1_1500154 Kit IQ-ELISA, MGC53867 Kit IQ-ELISA, pcna.L Kit IQ-ELISA, pcna1 Kit IQ-ELISA, LOC106420145 Kit IQ-ELISA, pcn1 Kit IQ-ELISA

ID gène: 5111

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy