Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Hexokinase 1 Kit ELISA

HK1 Reactivité: Humain Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
N° du produit ABIN6574201
  • Antigène Voir toutes Hexokinase 1 (HK1) Kits ELISA
    Hexokinase 1 (HK1)
    Reactivité
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    62.5 pg/mL - 4000 pg/mL
    Seuil minimal de détection
    62.5 pg/mL
    Application
    ELISA
    Fonction
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HK1 in human serum, plasma, tissue homogenates, cell lysates.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Type d'échantillon
    Cell Lysate, Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité
    This assay has high sensitivity and excellent specificity for detection of Hexokinase 1 (HK1)
    Réactivité croisée (Details)
    No significant cross-reactivity or interference between Hexokinase 1 (HK1) and analogues was observed.
    Sensibilité
    25.5 pg/mL
    Ingrédients
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
  • Commentaires

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Volume d'échantillon
    100 μL
    Durée du test
    3 h
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards,
    2. Add 100μL standard or sample to each well. Incubate 1 hours at 37 °C,
    3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37 °C,
    4. Aspirate and wash 3 times,
    5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
    6. Aspirate and wash 5 times,
    7. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
    8. Add 50μL Stop Solution. Read at 450nm immediately.
    Préparation des réactifs
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 16,000pg/mL. Firstly dilute the stock solution to 4,000pg/mL and the diluted standard serves as the highest standard (4,000pg/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 4,000pg/mL, 2,000pg/mL, 1,000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Préparation de l'échantillon
    • It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.
    • If the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.
    • If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.
    • Please estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).
    Précision du teste
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV < 10%
    Inter-Assay: CV < 12%
    Restrictions
    For Research Use only
  • Précaution d'utilisation
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
    2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.
    .
    Date de péremption
    6 months
  • Antigène Voir toutes Hexokinase 1 (HK1) Kits ELISA
    Hexokinase 1 (HK1)
    Abstract
    HK1 Produits
    Synonymes
    HK1-ta Kit ELISA, HK1-tb Kit ELISA, HK1-tc Kit ELISA, HKD Kit ELISA, HKI Kit ELISA, HMSNR Kit ELISA, HXK1 Kit ELISA, Hexokinase Kit ELISA, hk1 Kit ELISA, HEX Kit ELISA, HEX-1 Kit ELISA, HK Kit ELISA, ARABIDOPSIS THALIANA HEXOKINASE 1 Kit ELISA, ATHXK1 Kit ELISA, F19B15.160 Kit ELISA, F19B15_160 Kit ELISA, GIN2 Kit ELISA, GLUCOSE INSENSITIVE 2 Kit ELISA, HEXOKINASE Kit ELISA, hexokinase 1 Kit ELISA, im:7148527 Kit ELISA, wu:fc09d08 Kit ELISA, wu:fc16e02 Kit ELISA, wu:fc21e02 Kit ELISA, wu:fq14b11 Kit ELISA, zgc:55790 Kit ELISA, zgc:77618 Kit ELISA, hexokinase-1 Kit ELISA, HK1 Kit ELISA, BB404130 Kit ELISA, Hk-1 Kit ELISA, Hk1-s Kit ELISA, dea Kit ELISA, mHk1-s Kit ELISA, HEXOKIN Kit ELISA, Hxk1 Kit ELISA, NtHxK1 Kit ELISA, NtHxk Kit ELISA, hexokinase Kit ELISA, StHK1 Kit ELISA, hxk Kit ELISA, hk1-A Kit ELISA, hexokinase 1 Kit ELISA, hexokinase-1 Kit ELISA, Hexokinase 1 Kit ELISA, hexokinase 1, putative Kit ELISA, hexokinase 1 L homeolog Kit ELISA, HK1 Kit ELISA, hk1 Kit ELISA, LOC100136279 Kit ELISA, Hex1 Kit ELISA, HXK1 Kit ELISA, Hk1 Kit ELISA, TA19800 Kit ELISA, hxk1 Kit ELISA, LOC107797523 Kit ELISA, LOC102604144 Kit ELISA, hk1.L Kit ELISA
    UniProt
    P19367
    Pathways
    Carbohydrate Homeostasis, L'effet Warburg
Vous êtes ici:
Support technique