DKK1 Kit ELISA

N° du produit ABIN6719679

N° du produit (Fournisseur): koa0865

Ce kit ELISA Rat DKK1 est un kit ELISA Colorimetric conçu pour quantifier Rat DKK1.

Aperçu rapide pour DKK1 Kit ELISA (ABIN6719679)

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 46.9 pg/mL - 3000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit DKK1 Kit ELISA

Seuil minimal de détection: 46.9 pg/mL

N° du produit (Fournisseur): koa0865

Fournisseur: Rockland

Fonction: Rat DKK1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant rat DKK1. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 15 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL, 419.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL rat DKK1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each rat DKK1 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: S23-H270

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: DKK1 (Dickkopf Homolog 1 (DKK1))

Autre désignation: DKK1

Sujet:

Synonyms: Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, DKK1, DKK-1, hDkk 1, hDkk1, mDkk1, SK

Background: Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

ID gène: 293897

NCBI Accession: NP_001099820

UniProt: D3Z9J1

Pathways: Signalisation WNT, Regulation of Muscle Cell Differentiation, Positive Regulation of fat Cell Differentiation