WISP1 Kit ELISA

N° du produit ABIN6719711

Détail du produit WISP1 Kit ELISA

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CCN family member 4, CCN4, WISP1, WISP1c, WISP1i, WISP1tc, Wnt 1 inducible signaling pathway protein 1, Wnt-1 induced secreted protein, WNT1 induced secreted protein 1, WNT1 inducible signaling pathway protein 1, WNT1 inducible-signaling pathway protein 1

Background: WISP1(WNT1-Inducible Signaling Pathway Protein 1), also known as CCN4, is a matricellular protein that in humans is encoded by the WISP1 gene. WISP1 is induced by WNT1 and belongs to the CCN family, which includes connective tissue growth factor, cysteine-rich-61, and nephroblastoma overexpressed. By use of radiation hybrid mapping panels, the WISP1 was mapped to chromosome 8q24.1-q24.3, roughly 4 Mb distal to MYC. It found that 2 distinct systems demonstrated that WISP induction was associated with expression of WNT1. WISP1 genomic DNA was amplified in colon cancer cell lines and in human colon tumors, and its RNA was overexpressed in 84 % of tumors examined compared with patient-matched normal mucosa.

Gene Name: WISP1

Production: Natural and recombinant mouse WISP1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: T23-N367

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse WISP1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse WISP1 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Autre désignation: WISP1 - CCN4 (WISP1 Produits)

Synonymes: CCN4 Kit ELISA, WISP1c Kit ELISA, WISP1i Kit ELISA, WISP1tc Kit ELISA, WISP-1 Kit ELISA, WISP1 Kit ELISA, si:ch211-38f10.2 Kit ELISA, AW146261 Kit ELISA, Elm1 Kit ELISA, zgc:172276 Kit ELISA, WNT1 inducible signaling pathway protein 1 Kit ELISA, WNT1 inducible signaling pathway protein 1a Kit ELISA, WNT1-inducible-signaling pathway protein 1-like Kit ELISA, WNT1 inducible signaling pathway protein 1b Kit ELISA, WISP1 Kit ELISA, wisp1a Kit ELISA, Wisp1 Kit ELISA, LOC100627044 Kit ELISA, wisp1b Kit ELISA

ID gène: 22402

NCBI Accession: NP_061353

UniProt: O54775

Pathways: Signalisation WNT, Growth Factor Binding