BMP7 Kit ELISA
Aperçu rapide pour BMP7 Kit ELISA (ABIN6719712)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 31.2 pg/mL
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N° du produit (Fournisseur)
- koa0871
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Fournisseur
- Rockland
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Fonction
- Rat BMP-7 Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant rat BMP-7. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat BMP-7 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat bone tissue, cell culture supernates, serum or plasma((heparin, EDTA) to each empty well. It is recommended that each rat BMP-7 standard solution and each sample be measured in duplicate.
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Commentaires
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Standard: Expression system for standard: CHO, Immunogen sequence: S292-H430
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
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Autre désignation
- BMP-7
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Sujet
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Synonyms: BMP 7, BMP7, BMP-7, Bone morphogenetic protein 7, Eptotermin alfa, OP 1, OP1, OP1-, Osteogenic protein 2
Background: Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene. The protein encoded by this gene is a member of the TGF-β superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.
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ID gène
- 85272
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UniProt
- G3V6W8
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Pathways
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
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