FASL Kit ELISA

N° du produit ABIN6719714

Détail du produit FASL Kit ELISA

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Apoptosis (APO 1) antigen ligand 1, Apoptosis antigen ligand, APT1LG1, APTL, CD178, CD95 L, CD95 ligand, CD95L, CD95L protein, Fas antigen ligand, FASL, FASLG, TNF superfamily member 6, TNFSF6, Tumor necrosis factor (ligand) superfamily member 6, Tumor necrosis factor ligand superfamily member 6

Background: FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signaling pathway has been shown to participate in immune escape and tumor genesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

Gene Name: FASLG

Production: Natural and recombinant rat FAS-L. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q127-L278

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat FAS-L standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each rat FAS-L standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: FAS-L (FASL Produits)

Synonymes: ALPS1B Kit ELISA, APT1LG1 Kit ELISA, APTL Kit ELISA, CD178 Kit ELISA, CD95-L Kit ELISA, CD95L Kit ELISA, FASL Kit ELISA, TNFSF6 Kit ELISA, fasl Kit ELISA, Fas-L Kit ELISA, Faslg Kit ELISA, Tnfsf6 Kit ELISA, gld Kit ELISA, Apt1Lg1 Kit ELISA, Fasl Kit ELISA, FASLG Kit ELISA, zgc:162027 Kit ELISA, Fas ligand Kit ELISA, Fas ligand L homeolog Kit ELISA, Fas ligand (TNF superfamily, member 6) Kit ELISA, FASLG Kit ELISA, faslg.L Kit ELISA, Fasl Kit ELISA, Faslg Kit ELISA, faslg Kit ELISA

ID gène: 25385

NCBI Accession: NP_037040

UniProt: P36940

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity