ANGPTL3 Kit ELISA

N° du produit ABIN6719722

N° du produit (Fournisseur): koa0833

Kit ELISA Humain ANGPTL3, test Colorimetric pour la quantification de Humain ANGPTL3.

Aperçu rapide pour ANGPTL3 Kit ELISA (ABIN6719722)

Antigène: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit ANGPTL3 Kit ELISA

Seuil minimal de détection: 312 pg/mL

N° du produit (Fournisseur): koa0833

Fournisseur: Rockland

Fonction: Human ANGPTL3 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human ANGPTL3. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human ANGPTL3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human ANGPTL3 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: sf21, Immunogen sequence: S17-E460

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: ANGPTL3 (Angiopoietin-Like 3 (ANGPTL3))

Autre désignation: ANGPTL3

Sujet:

Synonyms: ANG 5, ANG-5, ANG5, Angiopoietin 5, Angiopoietin like 3, Angiopoietin related protein 3, Angiopoietin-5, Angiopoietin-like protein 3, Angiopoietin-related protein 3, ANGL3_HUMAN, ANGPT5, ANGPTL3, ANL3, FHBL2, OTTHUMP00000010719, UNQ153/PRO179

Background: ANGPTL3, also known as Angiopoietin-like 3 or HYPLl, is a protein that in humans is encoded by the ANGPTL3 gene. It is mapped to 1p31.3. The protein encoded by this gene is a member of the angiopoietin-like family of secreted factors. It is expressed predominantly in the liver, and has the characteristic structure of angiopoietins, consisting of a signal peptide, N-terminal coiled-coil domain, and the C-terminal fibrinogen (FBN)-like domain. ANGPTL3 also acts as dual inhibitor of lipoprotein lipase (LPL) and endothelial lipase (EL), and increases plasma triglyceride and HDL cholesterol in rodents. It can inhibit endothelial lipase to catalyze HDL-phospholipid and increase HDL-PL levels.

ID gène: 27329

NCBI Accession: NP_055310

UniProt: Q9Y5C1