PLAT Kit ELISA

N° du produit ABIN6719753

N° du produit (Fournisseur): koa0538

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain PLAT.

Aperçu rapide pour PLAT Kit ELISA (ABIN6719753)

Antigène: PLAT (Plasminogen Activator, Tissue (PLAT))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Détail du produit PLAT Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0538

Fournisseur: Rockland

Fonction: Human PLAT - TPA Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human PLAT. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human PLAT standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human PLAT standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: CHO, Immunogen sequence: M1-P562

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: PLAT (Plasminogen Activator, Tissue (PLAT))

Autre désignation: PLAT/TPA

Sujet:

Synonyms: Alteplase, Plasminogen activator tissue, Plasminogen activator tissue type, PLAT, Reteplase, t-PA, T-plasminogen activator, Tissue-type plasminogen activator chain B, tPA, TPA_HUMAN

Background: Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

ID gène: 5327

NCBI Accession: NP_000921

UniProt: P00750

Pathways: Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome