Midkine Kit ELISA

N° du produit ABIN6719771

Détail du produit Midkine Kit ELISA

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Amphiregulin-associated protein, ARAP, Mdk, Midgestation and kidney protein, Midkine, MK, MK_HUMAN, Neurite outgrowth-promoting factor 2, Neurite outgrowth-promoting protein

Background: Midkine(MK or MDK) also known as neurite growth-promoting factor 2(NEGF2) is a protein that in humans is encoded by the MDK gene. Midkine is a basic heparin-binding growth factor of low molecular weight, and forms a family with pleiotrophin(NEGF1, 46 % homologous with MK). It is a nonglycosylated protein, composed of two domains held by disulfide bridges. It is a developmentally important retinoic acid-responsive gene product strongly induced during mid-gestation, hence the name midkine. Restricted mainly to certain tissues in the normal adult, it is strongly induced during oncogenesis, inflammation and tissue repair. MK is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. A molecular complex containing receptor-type tyrosine phosphatase zeta(PTPζ), low density lipoprotein receptor-related protein(LRP1), anaplastic leukemia kinase(ALK) and syndecans is considered to be its receptor.

Gene Name: MDK

Production: Natural and recombinant human Midkine. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: K23-D143

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL human Midkine standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Midkine standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Other Performance Data: DOE: 2-8° C - 5/9/18 -20° C - 11/10/18

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Midkine (MDK) (Midkine (Neurite Growth-Promoting Factor 2) (MDK))

Autre désignation: Midkine (MDK Produits)

Synonymes: ARAP Kit ELISA, MK Kit ELISA, NEGF2 Kit ELISA, Mek Kit ELISA, MK-B Kit ELISA, PTF-alpha-1 Kit ELISA, mdk Kit ELISA, midkine Kit ELISA, negf2 Kit ELISA, RIHB Kit ELISA, cb518 Kit ELISA, fb79b05 Kit ELISA, fj33b02 Kit ELISA, fj48a12 Kit ELISA, mdk2 Kit ELISA, plei2 Kit ELISA, wu:fb79b05 Kit ELISA, wu:fj33b02 Kit ELISA, wu:fj48a12 Kit ELISA, MK-A Kit ELISA, PTF-alpha-2 Kit ELISA, X-PTF-alpha1 Kit ELISA, XMK Kit ELISA, mdk-a Kit ELISA, mdk-b Kit ELISA, midkine Kit ELISA, midkine L homeolog Kit ELISA, midkine (neurite growth-promoting factor 2) Kit ELISA, midkine b Kit ELISA, midkine S homeolog Kit ELISA, MDK Kit ELISA, Mdk Kit ELISA, mdk.L Kit ELISA, mdkb Kit ELISA, mdk.S Kit ELISA

ID gène: 4192

NCBI Accession: NP_001012333

UniProt: P21741

Pathways: Signalisation RTK, M Phase, Skeletal Muscle Fiber Development