Prostate Specific Antigen Kit ELISA
Aperçu rapide pour Prostate Specific Antigen Kit ELISA (ABIN6719774)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 312 pg/mL
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N° du produit (Fournisseur)
- koa0486
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Fournisseur
- Rockland
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Fonction
- Human Kallikrein 3 Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant human Kallikrein 3. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human Kallikrein 3 standard solution and each sample be measured in duplicate.
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Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: A18-P261
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- Prostate Specific Antigen (PSA)
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Autre désignation
- Kallikrein 3
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Sujet
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Synonyms: APS, Gamma seminoprotein, Gamma-seminoprotein, hK3, Kallikrein 3, Kallikrein related peptidase 3, Kallikrein-3, KLK 3, KLK2A1, KLK3, Klk3, KLK3_HUMAN, P 30 antigen, P-30 antigen, P30 antigen, Prostate Specific Antigen, Prostate-specific antigen, Psa, Semenogelase, Seminin
Background: Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland, PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3
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ID gène
- 354
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NCBI Accession
- NP_001025218
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UniProt
- P07288
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Pathways
- Système du Complément
Antigène Voir toutes Prostate Specific Antigen (PSA) Kits ELISA
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