VEGFB Kit ELISA

N° du produit ABIN6719783

N° du produit (Fournisseur): koa0852

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris VEGFB.

Aperçu rapide pour VEGFB Kit ELISA (ABIN6719783)

Antigène: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (heparin), Serum

Détail du produit VEGFB Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0852

Fournisseur: Rockland

Fonction: Mouse VEGF-B Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: Q20-A207 & Q20-R148

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 5.0%, 7.5%, 5.0%
Inter-Assay Precision (CV%): 7.0%, 8.2%, 5.7%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: VEGFB (Vascular Endothelial Growth Factor B (VEGFB))

Autre désignation: VEGF-B

Sujet:

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

Gene Full Name: vascular endothelial growth factor B

ID gène: 22340

NCBI Accession: NP_001172093

UniProt: P49766

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals