RAGE Kit ELISA

N° du produit ABIN6719817

N° du produit (Fournisseur): koa0495

Ce kit ELISA Souris RAGE est un kit ELISA Colorimetric conçu pour quantifier Souris RAGE.

Aperçu rapide pour RAGE Kit ELISA (ABIN6719817)

Antigène: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit RAGE Kit ELISA

Seuil minimal de détection: 78 pg/mL

N° du produit (Fournisseur): koa0495

Fournisseur: Rockland

Fonction: Mouse RAGE Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse RAGE. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL mouse RAGE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse RAGE standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: Q24-A342

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: RAGE (AGER) (Advanced Glycosylation End Product-Specific Receptor (AGER))

Autre désignation: RAGE

Sujet:

Synonyms: Advanced glycosylation end product specific receptor, Advanced glycosylation end product-specific receptor, Ager, DAMA 358M23.4, LE 9211 A antigen, MAPK MAK MRK overlapping kinase, MGC22357, MOK, OTTHUMP00000174885, RAGE 1, RAGE isoform NtRAGE-delta, RAGE isoform sRAGE-delta, RAGE_HUMAN, RAGE1, Receptor for advanced glycation end products, receptor for advanced glycation end-products variant 20, Receptor for advanced glycosylation end products, Renal tumor antigen, Renal tumor antigen 1

Background: RAGE, the Receptor for Advanced Glycation Endproducts, is a 35kD transmembrane receptor of the immunoglobulin super family. It is also known as ""AGER"". AGER gene is mapped to chromosome 6p21.3 by mapping by contiguous cosmids and YAC clones and by fluorescence in situ hybridization. The expression of RAGE is particularly increased in neurons close to deposits of amyloid beta peptide and to neurofibrillary tangles. RAGE has been linked to several chronic diseases, which are thought to result from vascular damage. The pathogenesis is hypothesized to include ligand binding upon which RAGE signals activation of the nuclear factor kappa B(NF-kappaB).

ID gène: 11596

NCBI Accession: NP_001258351

UniProt: Q62151

Pathways: Carbohydrate Homeostasis, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, S100 Proteins