TLR3 Kit ELISA
Aperçu rapide pour TLR3 Kit ELISA (ABIN6719884)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 156 pg/mL
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N° du produit (Fournisseur)
- koa0547
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Fournisseur
- Rockland
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Fonction
- Human TLR3 Sandwich ELISA Kit for Quantitative Detection
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Marque
- AccuSignal™
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Analytical Method
- Quantitative
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Specificité
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Production: Natural and recombinant human TLR3. There is no detectable cross-reactivity with other relevant proteins.
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Sensibilité
- < 10 pg/mL
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Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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Indications d'application
- Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human TLR3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human TLR3 standard solution and each sample be measured in duplicate.
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Commentaires
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Standard: Expression system for standard: NSO, Immunogen sequence: K27-S711
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Restrictions
- For Research Use only
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- TLR3 (Toll-Like Receptor 3 (TLR3))
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Autre désignation
- TLR3
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Sujet
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Synonyms: CD_antigen=CD283, CD283, IIAE2, Toll like receptor 3
Background: Toll-like receptor 3(TLR3) also known as CD283(cluster of differentiation 283) is a protein that in humans is encoded by the TLR3 gene. The TLR3 gene was mapped to chromosome 4q35 by fluorescence in situ hybridization. TLR3 specifically recognizes double-stranded RNA(dsRNA) and induces multiple intracellular events responsible for innate antiviral immunity against a number of viral infections.
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ID gène
- 7098
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NCBI Accession
- NP_003256
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UniProt
- O15455
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Pathways
- Signalisation TLR, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades
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