Kallikrein 11 Kit ELISA

N° du produit ABIN6719900

N° du produit (Fournisseur): koa0669

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Kallikrein 11.

Aperçu rapide pour Kallikrein 11 Kit ELISA (ABIN6719900)

Antigène: Kallikrein 11 (KLK11)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit Kallikrein 11 Kit ELISA

Seuil minimal de détection: 156 pg/mL

N° du produit (Fournisseur): koa0669

Fournisseur: Rockland

Fonction: Human KLK11 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: E19-N250

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK11 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4.9%, 7.9%, 4.9%
Inter-Assay Precision (CV%): 7.3%, 9.3%, 5.4%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Kallikrein 11 (KLK11)

Autre désignation: KLK11

Sujet:

Background: Kallikrein-11 is a protein that in humans is encoded by the KLK11 gene. Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in two transcript variants encoding two different isoforms which are differentially expressed.

Gene Full Name: kallikrein related peptidase 11

ID gène: 11012

NCBI Accession: NP_001129504

UniProt: Q9UBX7

Pathways: Système du Complément