Kallikrein 12 Kit ELISA

N° du produit ABIN6719901

Détail du produit Kallikrein 12 Kit ELISA

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: DKFZp686H1078, Kallikrein like protein 5, Kallikrein related peptidase 12, Kallikrein12, KLK 12, KLK L5, KLK12, KLKL5, MGC42603

Background: Kallikrein-12 is a protein that in humans is encoded by the KLK12 gene. It belongs to the kallikrein subgroup of serine proteases, which have diverse physiologic functions in many tissues. KLK12 is expressed primarily in salivary gland, stomach, uterus, trachea, prostate, thymus, lung, colon, brain, breast, and thyroid gland and at lower levels in testis, pancreas, small intestine, and spinal cord. The expression of KLK12 is downregulated at the mRNA level in breast cancer tissues and is upregulated by steroid hormones in breast and prostate cancer cell lines.

Gene Name: KLK12

Production: Natural and recombinant human KLK12. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A18-N248

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human KLK12 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, tissue homogenates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human KLK12 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Kallikrein 12 (KLK12) (Kallikrein-Related Peptidase 12 (KLK12))

Autre désignation: KLK12 (KLK12 Produits)

Synonymes: KLK12 Kit ELISA, 2310008B01Rik Kit ELISA, AI324852 Kit ELISA, KLK-L5 Kit ELISA, KLKL5 Kit ELISA, kallikrein-12 Kit ELISA, kallikrein related-peptidase 12 Kit ELISA, kallikrein related peptidase 12 Kit ELISA, LOC611766 Kit ELISA, Klk12 Kit ELISA, KLK12 Kit ELISA

ID gène: 43849

NCBI Accession: NP_062544

UniProt: Q9UKR0

Pathways: Système du Complément