CD13 Kit ELISA

N° du produit ABIN6719920

N° du produit (Fournisseur): koa0715

Kit ELISA Souris CD13, test Colorimetric pour la quantification de Souris CD13.

Aperçu rapide pour CD13 Kit ELISA (ABIN6719920)

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit CD13 Kit ELISA

Seuil minimal de détection: 312 pg/mL

N° du produit (Fournisseur): koa0715

Fournisseur: Rockland

Fonction: Mouse CD13 - Aminopeptidase N Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse CD13. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 312pg/mL-20,000pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL mouse CD13 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CD13 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: K69-S966

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Autre désignation: CD13/Aminopeptidase N

Sujet:

Synonyms: Alanyl (membrane) aminopeptidase, Alanyl aminopeptidase, Alanyl membrane aminopeptidase, Aminopeptidase M, Aminopeptidase N, AMPN_HUMAN, ANPEP, AP M, AP N, AP-M, AP-N, APN, CD 13, CD13, CD13 antigen, gp150, hAPN, Lap 1, Lap1, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, p150, PEPN

Background: Alanine aminopeptidase, also known as ANPEP or CD13. is an enzyme that is used as a biomarker to detect damage to the kidneys, and that may be used to help diagnose certain kidney disorders. It is mapped to 15q26.1. Aminopeptidase N is located in the small-intestinal and renal microvillar membrane, and also in other plasma membranes. In the small intestine, Aminopeptidase N plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases, and it is also thought to be involved in the metabolism of regulatory peptides by diverse cell types, including small intestinal and renal tubular epithelial cells, macrophages, granulocytes, and synaptic membranes from the CNS. Petrovic et al showed that CD13 was required for endothelial cell invasion in response to bradykinin. Inhibition of CD13 abrogated internalization of bradykinin receptor B2 and reduced endothelial cell motility.

ID gène: 16790

NCBI Accession: NP_032512

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones