CD25 Kit ELISA

N° du produit ABIN6719931

N° du produit (Fournisseur): koa0552

Le kit ELISA CD25 (ABIN6719931) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris CD25.

Aperçu rapide pour CD25 Kit ELISA (ABIN6719931)

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit CD25 Kit ELISA

Seuil minimal de détection: 78 pg/mL

N° du produit (Fournisseur): koa0552

Fournisseur: Rockland

Fonction: Mouse CD25 - IL-2sR alpha Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: E22-K236

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 7%, 7.3%, 5.2%
Inter-Assay Precision (CV%): 6.4%, 7.7%, 7%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25/IL-2sR alpha

Sujet:

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Gene Full Name: interleukin 2 receptor, alpha chain

ID gène: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation