CD25 Kit ELISA

N° du produit ABIN6719931

Détail du produit CD25 Kit ELISA

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: CD122, CD122 antigen, CD132, CD132 antigen, CD25, CD25 antigen, Cytokine receptor common gamma chain, Gamma C, High affinity IL 2 receptor subunit beta, IL 2 RA, IL 2 receptor, IL 2 receptor alpha subunit, IL 2R gamma chain, IL2R, IL2RA, IL2RB, IL2RG, IMD4, interleukin 2 receptor alpha, Interleukin 2 receptor alpha chain, interleukin 2 receptor beta, Interleukin 2 receptor beta chain, interleukin 2 receptor gamma, Interleukin 2 receptor gamma chain, p55, p64, P70 75, p75, SCIDX, SCIDX1, TAC antigen, TCGFR, XSCID

Background: CD25 is the alpha chain of the IL-2 receptor. It is a type I transmembrane protein present on activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes that associates with CD122 to form a heterodimer that can act as a high-affinity receptor for IL-2. CD25 has been used as a marker to identify CD4+FoxP3+ regulatory T cells in mice. By in situ hybridization, the IL2R gene was mapped to 10p15-p14. Its soluble form, called sIL-2R may be elevated in these diseases and is occasionally used to track disease progression.

Gene Name: IL2RA

Production: Natural and recombinant mouse CD25. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: E22-K236

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL, 78pg/mL mouse CD25 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin, EDTA) to each empty well. We recommend that each mouse CD25 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Other Performance Data: Expiration Dates: 4° C - 2/8/18 -20° C - 8/9/18

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

Autre désignation: CD25 - IL-2sR alpha (IL2RA Produits)

Synonymes: CD25 Kit ELISA, IDDM10 Kit ELISA, IL2R Kit ELISA, TCGFR Kit ELISA, IL2RAC Kit ELISA, IL-2RA Kit ELISA, Il2r Kit ELISA, Ly-43 Kit ELISA, interleukin 2 receptor subunit alpha Kit ELISA, interleukin-2 receptor subunit alpha-like Kit ELISA, interleukin 2 receptor, alpha chain Kit ELISA, IL2RA Kit ELISA, Il2ra Kit ELISA, LOC100222957 Kit ELISA

ID gène: 16184

NCBI Accession: NP_032393

UniProt: P01590

Pathways: Signalistation JAK/STAT, Growth Factor Binding, Activated T Cell Proliferation