IL-11 Kit ELISA

N° du produit ABIN6719999

N° du produit (Fournisseur): koa0233

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris IL-11.

Aperçu rapide pour IL-11 Kit ELISA (ABIN6719999)

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 7.8 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum

Détail du produit IL-11 Kit ELISA

Seuil minimal de détection: 7.8 pg/mL

N° du produit (Fournisseur): koa0233

Fournisseur: Rockland

Fonction: Mouse IL-11 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse IL-11. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 7.8 pg/mL-500 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse IL-11 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each mouse IL-11 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: P22-L199

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-11 (IL11) (Interleukin 11 (IL11))

Autre désignation: IL-11

Sujet:

Synonyms: Adipogenesis inhibitory factor, AGIF, IL 11, IL-11, Il11, IL11_HUMAN, Interleukin 11, Interleukin-11, Interleukin11, Oprelvekin

Background: Interleukin 11(IL-11) is a protein that in humans is encoded by the IL11 gene. The protein encoded by this gene is a member of the gp130 family of cytokines. This gene is mapped to 19q13.42. It is a key regulator of multiple events in hematopoiesis, most notably the stimulation of megakaryocyte maturation. IL-11 has been demonstrated to improve platelet recovery after chemotherapy-induced thrombocytopenia, induce acute phase proteins, modulate antigen-antibody responses, participate in the regulation of bone cell proliferation and differentiation and could be use as a therapeutic for osteoporosis. What's more, IL-11 can stimulate the growth of certain lymphocytes and it has functions in many other tissues, including the brain, gut, testis and bone. In transgenic mice, overexpression of the human IL11 gene resulted in the stimulation of bone formation to increase cortical thickness and strength of long bones.

ID gène: 16156

NCBI Accession: NP_032376

UniProt: P47873

Pathways: Signalistation JAK/STAT, Negative Regulation of Hormone Secretion