IL-15 Kit ELISA

N° du produit ABIN6720049

N° du produit (Fournisseur): koa0239

Kit ELISA Humain IL-15, test Colorimetric pour la quantification de Humain IL-15.

Aperçu rapide pour IL-15 Kit ELISA (ABIN6720049)

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit IL-15 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

N° du produit (Fournisseur): koa0239

Fournisseur: Rockland

Fonction: Human IL-15 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IL-15. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 3 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL human IL-15 standard solutions into the pre-coated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human IL-15 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: N49-S162

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL-15 (IL15) (Interleukin 15 (IL15))

Autre désignation: IL-15

Sujet:

Synonyms: IL 15, Interleukin 15, Interleukin15, MGC9721

Background: Interleukin(IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2(IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor. IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection. The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9KDa.

ID gène: 3600

NCBI Accession: NP_000576

UniProt: P40933

Pathways: Signalistation JAK/STAT, Glycosaminoglycan Metabolic Process