Angiopoietin 1 Kit ELISA

N° du produit ABIN6720094

Détail du produit Angiopoietin 1 Kit ELISA

Antigène: Angiopoietin 1 (ANGPT1)

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 1110046O21Rik, AGP 1, AGP1, AGPT, ANG 1, ANG1, Angiopoietin1, ANGPT 1, ANGPT1, KIAA0003

Background: Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Gene Name: ANGPT1

Production: Natural and recombinant rat Angiopoietin-1. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: H16-F498

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 313pg/mL, 156pg/mL rat Angiopoietin-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat Angiopoietin-1 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Angiopoietin 1 (ANGPT1)

Autre désignation: Angiopoietin-1 (ANGPT1 Produits)

Synonymes: AGP1 Kit ELISA, AGPT Kit ELISA, ANG1 Kit ELISA, 1110046O21Rik Kit ELISA, Ang-1 Kit ELISA, Ang1 Kit ELISA, Agpt Kit ELISA, Agpt1 Kit ELISA, ang1 Kit ELISA, wu:fc76g06 Kit ELISA, ANG-1 Kit ELISA, angpt1 Kit ELISA, MGC80788 Kit ELISA, angiopoietin 1 Kit ELISA, angiopoietin 1 S homeolog Kit ELISA, ANGPT1 Kit ELISA, Angpt1 Kit ELISA, angpt1 Kit ELISA, angpt1.S Kit ELISA

ID gène: 89807

NCBI Accession: NP_445998

UniProt: O35460

Pathways: Signalisation RTK, Glycosaminoglycan Metabolic Process