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Cardiotrophin 1 Kit ELISA

CTF1 Reactivité: Humain Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
N° du produit ABIN6720095
  • Antigène Voir toutes Cardiotrophin 1 (CTF1) Kits ELISA
    Cardiotrophin 1 (CTF1)
    Reactivité
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: Cardiophin 1, Cardiophin1, Cardiotrophin I, Cardiotrophin-1, CardiotrophinI, CT 1, CT-1, CT1, CTF 1, Ctf1, CTF1_HUMAN

    Background: Cardiotrophin-1(CT-1) is a member of the family of cytokines that includes leukemia inhibitory factor(LIF), ciliary neurotrophic factor(CNTF), oncostatin M(OSM), interleukin-6(IL6), and interleukin-11(IL11). And the CT-1 gene is mapped to 1p21-p13. The human CT-1 protein contains 201 amino acids and shares 80 % amino acid identity with the 203-amino acid mouse CT-1 sequence, however, unlike the mouse protein, human CT-1 has 2 rather than 1 cys and has no N-glycosylation site. Despite lacking a signal sequence, secreted CT-1 and mouse CT-1 induce cardiac myocyte hypertrophy in cell culture and bind to both mouse and human LIFR but not to OSMR. Furthermore, A 1.7-kb CT-1 transcript was detected at high levels in heart, skeletal muscle, prostate, and ovary. Low levels were detected in lung, kidney, pancreas, thymus, testis, and small intestine, with little or no expression detected in brain, placenta, spleen, colon, and peripheral blood leukocytes. And it was also observed strong expression in fetal lung and kidn...

    Gene Name: CTF1

    Production: Natural and recombinant human CT-1. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: S2-A201

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CT-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CT-1 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 156pg/mL-10000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes Cardiotrophin 1 (CTF1) Kits ELISA
    Cardiotrophin 1 (CTF1)
    Autre désignation
    Cardiotrophin-1 (CTF1 Produits)
    Synonymes
    CTF1 Kit ELISA, ct1 Kit ELISA, ct-1 Kit ELISA, ctf2 Kit ELISA, CT-1 Kit ELISA, CT1 Kit ELISA, cardiotrophin 1 Kit ELISA, cardiotrophin-1 Kit ELISA, CTF1 Kit ELISA, ctf1 Kit ELISA, Ctf1 Kit ELISA, LOC100736818 Kit ELISA
    ID gène
    1489
    NCBI Accession
    NP_001136016
    UniProt
    Q16619
    Pathways
    Signalistation JAK/STAT
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