Angiopoietin 2 Kit ELISA

N° du produit ABIN6720122

Détail du produit Angiopoietin 2 Kit ELISA

Antigène: Angiopoietin 2 (ANGPT2)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AGPT2, ANG 2, ANG-2, ANG2, Angiopoietin 2a, Angiopoietin 2B, Angiopoietin-2, Angiopoietin2, ANGP2_HUMAN, ANGPT 2, Angpt2, Tie2 ligand

Background: Angiopoietin-2 is a protein that in humans is encoded by the ANGPTL2 gene. Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. Human and mouse ANGPT2 are 85 % identical in amino acid sequence and approximately 60 % identical to their ANGPT1 homologs. Transgenic overexpression of ANGPT2 in mice disrupted blood vessel formation in the mouse embryo. By FISH and radiation hybrid analysis, the human ANGPT2 gene was mapped to 8p23, and in a region of syntenic homology on mouse chromosome 8, where the Angpt2 gene was mapped. ANGPT2 is a naturally occurring antagonist of angiopoietin-1 that competes for binding to the TIE2 receptor and blocks ANGPT1-induced TIE2 autophosphorylation during vasculogenesis .

Gene Name: ANGPT2

Production: Natural and recombinant mouse Angiopoietin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: M1-F496

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse Angiopoietin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Angiopoietin-2 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Angiopoietin 2 (ANGPT2)

Autre désignation: Angiopoietin-2 (ANGPT2 Produits)

Synonymes: AGPT2 Kit ELISA, ANG2 Kit ELISA, Agpt2 Kit ELISA, Ang-2 Kit ELISA, Ang2 Kit ELISA, ang2 Kit ELISA, angpt2 Kit ELISA, ANGIOPOIETIN-2 Kit ELISA, ANG-2 Kit ELISA, ANGPT2 Kit ELISA, Angrp Kit ELISA, Rnase5b Kit ELISA, ANG3 Kit ELISA, C11orf2 Kit ELISA, C11orf3 Kit ELISA, FFR Kit ELISA, angiopoietin 2 Kit ELISA, angiopoietin 2b Kit ELISA, angiogenin, ribonuclease A family, member 2 Kit ELISA, VPS51, GARP complex subunit Kit ELISA, ANGPT2 Kit ELISA, Angpt2 Kit ELISA, angpt2b Kit ELISA, Ang2 Kit ELISA, VPS51 Kit ELISA

ID gène: 11601

NCBI Accession: NP_031452

UniProt: B9EHQ4

Pathways: Signalisation RTK