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S100A8 Kit ELISA

S100A8 Reactivité: Souris Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
N° du produit ABIN6720149
  • Antigène Voir toutes S100A8 Kits ELISA
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Reactivité
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: 60B8AG, CAGA, Calgranulin-A, Calprotectin L1L subunit, CFAG, CGLA, CP-10, Cystic fibrosis antigen, L1Ag, Leukocyte L1 complex light chain, MA387, MIF, Migration inhibitory factor-related protein 8, MRP 8, MRP-8, MRP8, Myeloid-related protein 8, NIF, p8, Protein S100-A8, S100 calcium binding protein A8, S100 calcium-binding protein A8 (calgranulin A), S100 calcium-binding protein A8, S100A8, S10A8_HUMAN, Urinary stone protein band A

    Background: S100 calcium-binding protein A8 (S100A8), also known as calgranulin A, is a protein that in humans is encoded by the S100A8 gene. It is mapped to chromosome 1q21.3 based on an alignment of the S100A8 sequence with the genomic sequence. The proteins S100A8 and S100A9 form a heterodimer called calprotectin which is a major calcium- and zinc-binding protein in the cytosol of neutrophils, monocytes, and keratinocytes. This gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. And S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis.

    Gene Name: S100A8

    Production: Natural and recombinant mouse S100A8. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: E.coli, Immunogen sequence: P2-E89

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse S100A8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse S100A8 standard solution and each sample be measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2 pg/mL-2000pg/mL

    Other Performance Data: DOE: 2-8° C - 04/12/19 -20° C - 10/12/19

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes S100A8 Kits ELISA
    S100A8 (S100 Calcium Binding Protein A8 (S100A8))
    Autre désignation
    S100A8 (S100A8 Produits)
    Synonymes
    60B8AG Kit ELISA, CAGA Kit ELISA, CFAG Kit ELISA, CGLA Kit ELISA, CP-10 Kit ELISA, L1Ag Kit ELISA, MA387 Kit ELISA, MIF Kit ELISA, MRP8 Kit ELISA, NIF Kit ELISA, P8 Kit ELISA, Mrp8 Kit ELISA, S100A8 Kit ELISA, 60B8Ag Kit ELISA, AI323541 Kit ELISA, B8Ag Kit ELISA, CFAg Kit ELISA, Caga Kit ELISA, p8 Kit ELISA, MRP-8 Kit ELISA, S100 calcium binding protein A8 Kit ELISA, S100 calcium binding protein A8 (calgranulin A) Kit ELISA, S100A8 Kit ELISA, S100a8 Kit ELISA
    ID gène
    20201
    NCBI Accession
    NP_038678
    UniProt
    P27005
    Pathways
    Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins
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