IL1RN Kit ELISA

N° du produit ABIN6720174

N° du produit (Fournisseur): koa0461

Le kit ELISA IL1RN (ABIN6720174) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain IL1RN.

Aperçu rapide pour IL1RN Kit ELISA (ABIN6720174)

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit IL1RN Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0461

Fournisseur: Rockland

Fonction: Human IL-1RA Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human IL-1RA. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL human IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human IL-1RA standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: R26-E177

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Autre désignation: IL-1RA

Sujet:

Synonyms: Anakinra, DIRA, CIL 1RA, ICIL-1RA, IL 1ra, IL 1RN, IL-1ra, IL-1RN, IL1 inhibitor, IL1F3, IL1RA, IL1RA_HUMAN, IL1RN, Interleukin 1 receptor antagonist, Interleukin 1 receptor antagonist protein, Interleukin-1 receptor antagonist protein, Intracellular IL 1 receptor antagonist type II, Intracellular interleukin 1 receptor antagonist, IRAP, MGC10430, MVCD4, Type II interleukin 1 receptor antagonist

Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization.3 IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

ID gène: 3557

NCBI Accession: NP_000568

UniProt: P18510

Pathways: Signalisation NF-kappaB, Hormone Transport, Cancer Immune Checkpoints