GAS6 Kit ELISA

N° du produit ABIN6720195

N° du produit (Fournisseur): koa0695

Le kit ELISA GAS6 (ABIN6720195) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris GAS6.

Aperçu rapide pour GAS6 Kit ELISA (ABIN6720195)

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit GAS6 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0695

Fournisseur: Rockland

Fonction: Mouse GAS6 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: D115-P673

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Commentaires:

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse GAS6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse GAS6 standard solution and each sample be measured in duplicate.

Précision du teste: Intra-Assay Precision (CV%): 4%, 6.1%, 5.7%
Inter-Assay Precision (CV%): 5%, 6.4%, 5.9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: GAS6 (Growth Arrest-Specific 6 (GAS6))

Autre désignation: GAS6

Sujet:

Background: GAS6 (Growth arrest-specific 6), also known as AXLLG or AXSF, is a human gene coding for the Gas6 protein. It is mapped to 13q34. GAS6 was originally found as a gene upregulated by growth arrested fibroblasts. This gene product is a gamma-carboxyglutamic acid (Gla)-containing protein which thought to be involved in the stimulation of cell proliferation, and may play a role in thrombosis. What's more, the product of the GAS6 gene, a ligand for the AXL, MER, and TYRO3 tyrosine kinase receptors, is a vitamin K-dependent protein, structurally related to anticoagulant protein S but lacking its anticoagulant activity. This protein also shows great importance in the cardiovascular system.

Gene Full Name: growth arrest specific 6

ID gène: 14456

NCBI Accession: NP_062394

UniProt: Q61592

Pathways: Signalisation RTK, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response