S100A9 Kit ELISA

N° du produit ABIN6720206

N° du produit (Fournisseur): koa0662

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Souris S100A9.

Aperçu rapide pour S100A9 Kit ELISA (ABIN6720206)

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit S100A9 Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

N° du produit (Fournisseur): koa0662

Fournisseur: Rockland

Fonction: Mouse S100A9 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse S100A9. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse S100A9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse S100A9 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: A2-K113

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: S100A9 (S100 Calcium Binding Protein A9 (S100A9))

Autre désignation: S100A9

Sujet:

Synonyms: Leukocyte L1 complex heavy chain, CAGB, Calgranulin B, Calprotectin L1H subunit, CFAG, CGLB, Cystic fibrosis antigen, Cystic fibrosis antigen B, L1AG, Leukocyte L1 complex heavy chain, LIAG, MAC 387, MAC387, MIF, Migration inhibitory factor related protein 14, Migration inhibitory factor-related protein 14, MRP14, Myeloid-related protein 14, NIF, P14, S100 A9, S100 calcium binding protein A9, S100 calcium binding protein A9 calgranulin B, S100 calcium-binding protein A9, S10A9_HUMAN

Background: Protein S100A9 also known as migration inhibitory factor-related protein 14 (MRP-14) or calgranulin-B is a protein that is encoded by the S100A9 gene. S100A9 is a member of the S100 family of proteins containing 2 EF hand calcium-binding motifs. It is mapped to Chromosome 3 in mouse. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. This protein may function in the inhibition of casein kinase. S100A9 complexes with S100A8, another member of the S100 family of calcium-modulated proteins regulates myeloid cell function by binding to Toll-like receptor-4 (TLR-4) and the receptor for advanced glycation end products.

ID gène: 20202

NCBI Accession: NP_001268781

UniProt: P31725

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteins