IL1RN Kit ELISA

N° du produit ABIN6720253

Détail du produit IL1RN Kit ELISA

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: ICIL 1RA, IL1 inhibitor, ICIL-1RA, ICIL1RA, IL-1ra, IL-1RN, IL1 inhibitor, IL1F3, IL1RA_HUMAN, IL1RN, Interleukin 1 Receptor Antagonist Protein, IRAP

Background: The interleukin-1 receptor antagonist(IL-1RA) is a protein that in humans is encoded by the IL1RN gene. The human IL1RN gene was localized to the long arm of chromosome 2 at band 2q14.2 by fluorescence in situ hybridization. IL-1RA is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha(IL1A) and interleukin 1, beta(IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses.

Gene Name: IL1RN

Production: Natural and recombinant mouse IL-1RA. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: R27-Q178

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse IL-1RA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse IL-1RA standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IL1RN (Interleukin 1 Receptor Antagonist (IL1RN))

Autre désignation: IL-1RA - IL1RN (IL1RN Produits)

Synonymes: DIRA Kit ELISA, ICIL-1RA Kit ELISA, IL-1RN Kit ELISA, IL-1ra Kit ELISA, IL-1ra3 Kit ELISA, IL1F3 Kit ELISA, IL1RA Kit ELISA, IRAP Kit ELISA, MVCD4 Kit ELISA, IRAP1 Kit ELISA, IL1RN Kit ELISA, IL-1RA Kit ELISA, F630041P17Rik Kit ELISA, interleukin 1 receptor antagonist Kit ELISA, interleukin 1 receptor antagonist-like Kit ELISA, IL1RN Kit ELISA, Il1rn Kit ELISA, IL1RNL Kit ELISA

ID gène: 16181

NCBI Accession: NP_001034790

UniProt: P25085

Pathways: Signalisation NF-kappaB, Hormone Transport, Cancer Immune Checkpoints