P-Selectin Kit ELISA

N° du produit ABIN6720280

N° du produit (Fournisseur): koa0301

Kit ELISA Humain P-Selectin, test Colorimetric pour la quantification de Humain P-Selectin.

Aperçu rapide pour P-Selectin Kit ELISA (ABIN6720280)

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Détail du produit P-Selectin Kit ELISA

Seuil minimal de détection: 62.5 pg/mL

N° du produit (Fournisseur): koa0301

Fournisseur: Rockland

Fonction: Human P-Selectin Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human sP-Selectin. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Selectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. It is recommended that each human P-Selectin standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: W42-A771

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Autre désignation: P-Selectin

Sujet:

Synonyms: Antigen CD62, CD62, CD62 antigen like family member P, GMP140, Granule membrane protein 140, Granule membrane protein 140 kDa, GRMP, LECAM3, Leukocyte endothelial cell adhesion molecule 3, Leukocyte-endothelial cell adhesion molecule 3, LYAM3_HUMAN, P selectin, P-selectin, PADGEM, Platelet activation dependent granule external membrane protein, Platelet activation dependent granule-external membrane protein, Platelet alpha granule membrane protein, PSEL, Selectin P

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.

ID gène: 6403

NCBI Accession: NP_002996

UniProt: P16109