Adipsin Kit ELISA

N° du produit ABIN6720317

Détail du produit Adipsin Kit ELISA

Antigène: Adipsin (CFD) (Complement Factor D (CFD))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 312 pg/mL - 20000 pg/mL

Seuil minimal de détection: 312 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Urine

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Adipsin, ADN, C3 convertase activator, CFAD_HUMAN, CFD, Complement factor D, Complement factor D preproprotein, D component of complement, DF, PFD, Properdin factor D

Background: Complement factor D(CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What's more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis.

Gene Name: CFD

Production: Natural and recombinant human CFD . There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: I26-A253

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 20,000pg/mL, 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human CFD standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma (heparin, EDTA) or urine to each empty well. We recommend that each human CFD standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 312pg/mL-20,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Adipsin (CFD) (Complement Factor D (CFD))

Autre désignation: CFD (CFD Produits)

Synonymes: ADIPSIN Kit ELISA, ADN Kit ELISA, DF Kit ELISA, PFD Kit ELISA, Adn Kit ELISA, Df Kit ELISA, EVE Kit ELISA, CFD Kit ELISA, adipsin Kit ELISA, pfd Kit ELISA, cfdl Kit ELISA, wu:fb61f12 Kit ELISA, zgc:109940 Kit ELISA, 10.5 Kit ELISA, 10.9 Kit ELISA, 14.10 Kit ELISA, 20.35 Kit ELISA, CG2328 Kit ELISA, Dmel\\CG2328 Kit ELISA, E(eve) Kit ELISA, Eve Kit ELISA, F Kit ELISA, V Kit ELISA, VI Kit ELISA, eve2 Kit ELISA, even Kit ELISA, l(2)46CFg Kit ELISA, l(2)46CFh Kit ELISA, l(2)46CFj Kit ELISA, l(2)46CFp Kit ELISA, l(2)46Ce Kit ELISA, l(2)46Cg Kit ELISA, complement factor D Kit ELISA, complement factor D (adipsin) Kit ELISA, complement factor D (adipsin) L homeolog Kit ELISA, even skipped Kit ELISA, CFD Kit ELISA, Cfd Kit ELISA, cfd.L Kit ELISA, cfd Kit ELISA, eve Kit ELISA

ID gène: 1675

NCBI Accession: NP_001304264

UniProt: P00746

Pathways: Système du Complément