Clusterin Kit ELISA

N° du produit ABIN6720361

Détail du produit Clusterin Kit ELISA

Antigène: Clusterin (CLU)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Seuil minimal de détection: 0.78 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AAG4, Aging associated protein 4, APO J, APOJ, CLI, CLU, CLU1, CLU2, Clusterin, Complement associated protein SP 40,40, Complement cytolysis inhibitor, Complement lysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, SP40, Sulfated glycoprotein 2, Testosterone repressed prostate message 2, TRPM 2, TRPM2

Background: Clusterin(apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms: dimeric acidic glycoprotein(DAG protein), testosterone repressed prostate message-2(TRPM-2), sulfated glycoprotein-2(SGP-2) and complement lysis inhibitor(CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic(H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Gene Name: CLU

Production: Natural and recombinant human Clusterin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D23-R227(beta)&S228-E449(alpha)

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL, 0.78 ng/mL human Clusterin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva or urine to each empty well. We recommend that each human Clusterin standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 0.78 ng/mL-50 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Clusterin (CLU)

Autre désignation: Clusterin (CLU Produits)

Synonymes: LOC100136120 Kit ELISA, clu Kit ELISA, MGC53055 Kit ELISA, zgc:73352 Kit ELISA, xclu Kit ELISA, MGC89163 Kit ELISA, CLU Kit ELISA, APO-J Kit ELISA, APOJ Kit ELISA, CLI Kit ELISA, CLU1 Kit ELISA, CLU2 Kit ELISA, KUB1 Kit ELISA, NA1/NA2 Kit ELISA, SGP-2 Kit ELISA, SGP2 Kit ELISA, SP-40 Kit ELISA, TRPM-2 Kit ELISA, TRPM2 Kit ELISA, DAG Kit ELISA, RATTRPM2B Kit ELISA, SP40 Kit ELISA, TRPM2B Kit ELISA, Trpm2 Kit ELISA, Trpmb Kit ELISA, AI893575 Kit ELISA, ApoJ Kit ELISA, Cli Kit ELISA, D14Ucla3 Kit ELISA, Sgp-2 Kit ELISA, Sgp2 Kit ELISA, Sugp-2 Kit ELISA, GP80 Kit ELISA, gpIII Kit ELISA, 40 Kit ELISA, clusterin-1 Kit ELISA, clusterin L homeolog Kit ELISA, clusterin Kit ELISA, Clusterin Kit ELISA, LOC100136120 Kit ELISA, clu.L Kit ELISA, clu Kit ELISA, CLU Kit ELISA, clus Kit ELISA, Clu Kit ELISA, LOC101113728 Kit ELISA

ID gène: 1191

NCBI Accession: NP_001822

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling