APOE Kit ELISA

N° du produit ABIN6720363

Détail du produit APOE Kit ELISA

Antigène: APOE (Apolipoprotein E (APOE))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 3.12 ng/mL - 200 ng/mL

Seuil minimal de détection: 3.12 ng/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Milk, Plasma (EDTA - heparin), Saliva, Serum, Urine

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AD2, Alzheimer disease 2, Apo E, Apo-E, ApoE, APOEA, Apolipoprotein E, Apolipoprotein-E, ApolipoproteinE, Apoprotein, LDLCQ5, LPG

Background: Apolipoprotein E (APOE) is a class of apolipoprotein found in the chylomicron and Intermediate-density lipoprotein (IDLs) that is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. In peripheral tissues, APOE is primarily produced by the liver and macrophages, and mediates cholesterol metabolism in an isoform- dependent manner. In the central nervous system, APOE is mainly produced by astrocytes, and transports cholesterol to neurons via APOE receptors, which are members of the low density lipoprotein receptor gene family. This protein is involved in Alzheimer's disease and cardiovascular disease.

Gene Name: APOE

Production: Natural human APOE. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: K19-H317

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL human APOE standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), saliva, milk or urine to each empty well. It is recommended that each human APOE standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 3.12 ng/mL-200 ng/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: APOE (Apolipoprotein E (APOE))

Autre désignation: APOE (APOE Produits)

Synonymes: ad2 Kit ELISA, apoprotein Kit ELISA, im:7036787 Kit ELISA, wu:fb69a05 Kit ELISA, zgc:110064 Kit ELISA, apoe Kit ELISA, AI255918 Kit ELISA, AD2 Kit ELISA, LDLCQ5 Kit ELISA, LPG Kit ELISA, APOEA Kit ELISA, Apo-E Kit ELISA, apolipoprotein E Kit ELISA, apolipoprotein Ea Kit ELISA, apoe Kit ELISA, apoea Kit ELISA, Apoe Kit ELISA, APOE Kit ELISA

ID gène: 348

NCBI Accession: NP_000032

UniProt: P02649

Pathways: Regulation of Cell Size, Lipid Metabolism