ADA Kit ELISA
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- Antigène Voir toutes ADA Kits ELISA
- ADA (Adenosine Deaminase (ADA))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- Sandwich ELISA for Quantitative Detection of Antigen
- Type d'échantillon
- Cell Culture Supernatant, Pleural Fluid, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- Attributs du produit
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Synonyms: ada, ADA1, ADA 1, Adenosine aminohydrolase, Adenosine deaminase
Background: Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Gene Name: ADA
Production: Natural and recombinant human ADA. There is no detectable cross-reactivity with other relevant proteins.
Standard: Expression system for standard: sf21, Immunogen sequence: A2-L363
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- Discover our top product ADA Kit ELISA
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- Indications d'application
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Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human ADA standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, tissue homogenates or pleural fluid to each empty well. It is recommended that each human ADA standard solution and each sample be measured in duplicate.
ELISA Dilution: 15.6pg/mL-1000pg/mL
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
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- Antigène Voir toutes ADA Kits ELISA
- ADA (Adenosine Deaminase (ADA))
- Autre désignation
- ADA (ADA Produits)
- Synonymes
- ADA-like Kit ELISA, xada Kit ELISA, ADA Kit ELISA, CG11994 Kit ELISA, Dmel\\CG11994 Kit ELISA, DrosADA Kit ELISA, dADA Kit ELISA, zgc:92028 Kit ELISA, adenosine deaminase Kit ELISA, adenosine deaminase S homeolog Kit ELISA, Adenosine deaminase Kit ELISA, ADA Kit ELISA, Ada Kit ELISA, ada.S Kit ELISA, ada Kit ELISA
- ID gène
- 100
- NCBI Accession
- NP_000013
- UniProt
- P00813
- Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Ribonucleoside Biosynthetic Process
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