Angiostatin Kit ELISA

N° du produit ABIN6720398

Détail du produit Angiostatin Kit ELISA

Antigène: Angiostatin (ANG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Ang K1-3

Background: Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity(ED₅₀ =70 nM) relative to kringles 1-4(ED₅₀ = 135 nM).

Gene Name: PLG

Production: Natural and recombinant human Angiostatin K1-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: V98-S355(K1-3)

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Angiostatin K1-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human serum, plasma or cell culture supernates to each empty well. It is recommended that each human Angiostatin K1-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Angiostatin (ANG)

Autre désignation: Angiostatin K1-3 (ANG Produits)

Synonymes: ALS9 Kit ELISA, HEL168 Kit ELISA, RNASE4 Kit ELISA, RNASE5 Kit ELISA, LPA Kit ELISA, AI649309 Kit ELISA, Pg Kit ELISA, Ab1-346 Kit ELISA, AI385586 Kit ELISA, Ang1 Kit ELISA, Rnase5 Kit ELISA, Rnase5a Kit ELISA, angiogenin Kit ELISA, plasminogen Kit ELISA, angiogenin, ribonuclease, RNase A family, 5 Kit ELISA, ANG Kit ELISA, PLG Kit ELISA, Plg Kit ELISA, Ang Kit ELISA

ID gène: 5340

NCBI Accession: NP_000292

UniProt: P00747

Pathways: Lipid Metabolism