Complement Factor H Kit ELISA

N° du produit ABIN6730142

Détail du produit Complement Factor H Kit ELISA

Antigène: Complement Factor H (CFH)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Complement Factor H ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse Complement Factor H.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Complement Factor H (CFH)

Autre désignation: Complement Factor H (CFH Produits)

Synonymes: AHUS1 Kit ELISA, AMBP1 Kit ELISA, ARMD4 Kit ELISA, ARMS1 Kit ELISA, CFHL3 Kit ELISA, FH Kit ELISA, FHL1 Kit ELISA, HF Kit ELISA, HF1 Kit ELISA, HF2 Kit ELISA, HUS Kit ELISA, Mud-1 Kit ELISA, NOM Kit ELISA, Sas-1 Kit ELISA, Sas1 Kit ELISA, Fh Kit ELISA, CFH Kit ELISA, im:7147412 Kit ELISA, si:ch211-207o17.2 Kit ELISA, fH Kit ELISA, armd4 Kit ELISA, arms1 Kit ELISA, cfh Kit ELISA, cfhl3 Kit ELISA, fhl1 Kit ELISA, hf1 Kit ELISA, hf2 Kit ELISA, hus Kit ELISA, complement factor H Kit ELISA, complement component factor h Kit ELISA, complement factor H-related protein 5 Kit ELISA, complement factor H L homeolog Kit ELISA, CFH Kit ELISA, Cfh Kit ELISA, cfh Kit ELISA, LOC100399888 Kit ELISA, LOC100733902 Kit ELISA, cfh.L Kit ELISA

UniProt: P06909

Pathways: Système du Complément, Cellular Response to Molecule of Bacterial Origin