Ephrin B2 Kit ELISA

N° du produit ABIN6730158

Kit ELISA Ephrin B2 Souris, Colorimetric test pour la quantification de Souris Ephrin B2.

Aperçu rapide pour Ephrin B2 Kit ELISA (ABIN6730158)

Antigène: Ephrin B2 (EFNB2)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.14 ng/mL - 200 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit Ephrin B2 Kit ELISA

Seuil minimal de détection: 0.14 ng/mL

Fonction: Mouse Ephrin-B2 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: Mouse BTLA, BTN1A1, C1ra, Cathepsin L, CD200 R1, CD2F-10, CD38, Cerberus 1, CRISP-4, CXCL17, DCC, DcTRAIL R2, Dkk-2, EG-VEGF, EPCR, EphA3, EphA4, Ephrin-B1, Frizzled-2, Galectin-4, Galectin-9, GDF-5, GDF-9, GFR alpha -2, GHR, HIN-1, ICAM-5, IGFBP-L1, IL-10 R alpha, IL17 RD, IL-22 R alpha 1, IL-3 R alpha, JAM-B, Latexin, LILRC1, Matrilin-4, Netrin-1, Netrin-4, Noggin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: Ephrin B2 (EFNB2)

Autre désignation: Ephrin-B2

Sujet: Gene Symbols: Ephrin-B2 (ELF-2) (EPH-related receptor tyrosine kinase ligand 5) (LERK-5) (HTK ligand) (HTK-L)

UniProt: P52800

Pathways: Signalisation RTK, Regulation of Muscle Cell Differentiation