Ephrin B1 Kit ELISA (EFNB1)

Details for Product EFNB1 Kit ELISA No. ABIN6730161
Antigène
Reactivité
Souris
Alternatives
Kits with alternative reactivity to:
6
4
3
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Options
Fonction Mouse Ephrin-B1 ELISA Kit.
Type d'échantillon Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytical Method Quantitative
Méthode de détection Colorimetric
Specificité This ELISA antibody pair recognizes Mouse Ephrin-B1.
Attributs du produit
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Ingrédients
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Matériel non inclus
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Antigène
Autre désignation Ephrin-B1 (EFNB1 ELISA Kit Extrait)
UniProt P52795
Pathways Signalisation RTK
Indications d'application Optimal working dilution should be determined by the investigator.
Protocole
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Stockage commentaire The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption 6 months