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Calcineurin B Kit ELISA (CAN) Kit ELISA

CAN Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant
N° du produit ABIN6730337
$796.95
Plus shipping costs $45.00
96 tests
local_shipping Destination: Etats-Unis
Envoi sous 21 à 31 jours ouvrables
  • Antigène
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Calcineurin B ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Calcineurin B.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène
    Autre désignation
    Calcineurin B (CAN ELISA Kit Extrait)
    Synonymes
    PPP3RL, BcDNA:RH11383, CG4209, CNB, Can, CnB, Dmel\\CG4209, canB-4F, dCnB1, Cnb, Cblp, CaNB2, protein phosphatase 3 regulatory subunit B, beta, Calcineurin B, calcineurin B, protein phosphatase 3, regulatory subunit B, beta, protein phosphatase 3 regulatory subunit B, alpha, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II), PPP3R2, CanB, Cnb, Ppp3r2, PPP3R1
    UniProt
    Q96LZ3
    Pathways
    Cellular Glucan Metabolic Process, VEGF Signaling
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