Calcineurin B Kit ELISA (CAN)

N° du produit ABIN6730337

Détail du produit Calcineurin B Kit ELISA

Antigène:

Calcineurin B (CAN)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Calcineurin B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Calcineurin B.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène:

Calcineurin B (CAN)

Autre désignation: Calcineurin B (CAN ELISA Kit Extrait)

Synonymes: PPP3RL, BcDNA:RH11383, CG4209, CNB, Can, CnB, Dmel\\CG4209, canB-4F, dCnB1, Cnb, Cblp, CaNB2, protein phosphatase 3 regulatory subunit B, beta, Calcineurin B, calcineurin B, protein phosphatase 3, regulatory subunit B, beta, protein phosphatase 3 regulatory subunit B, alpha, protein phosphatase 3, regulatory subunit B, alpha isoform (calcineurin B, type II), PPP3R2, CanB, Cnb, Ppp3r2, PPP3R1

UniProt: Q96LZ3

Pathways: Cellular Glucan Metabolic Process, VEGF Signaling