ACLY Kit ELISA

N° du produit ABIN6730345

Détail du produit ACLY Kit ELISA

Antigène: ACLY (ATP Citrate Lyase (ACLY))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human ACLY ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human ACLY.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: ACLY (ATP Citrate Lyase (ACLY))

Autre désignation: ACLY (ACLY Produits)

Synonymes: ACLY Kit ELISA, DDBDRAFT_0205389 Kit ELISA, DDBDRAFT_0235360 Kit ELISA, DDB_0205389 Kit ELISA, DDB_0235360 Kit ELISA, ACL Kit ELISA, BcDNA:LD21334 Kit ELISA, CG8322 Kit ELISA, DmATPCL Kit ELISA, Dmel\\CG8322 Kit ELISA, anon-WO0140519.179 Kit ELISA, dATPCL Kit ELISA, l(2)01466 Kit ELISA, l(2)k09217 Kit ELISA, n(2)k09217 Kit ELISA, ATPCL Kit ELISA, CLATP Kit ELISA, A730098H14Rik Kit ELISA, AW538652 Kit ELISA, Clatp Kit ELISA, acly Kit ELISA, cb722 Kit ELISA, zgc:92008 Kit ELISA, ATP citrate lyase Kit ELISA, ATP citrate lyase S homeolog Kit ELISA, ATP-citrate synthase Kit ELISA, citrate synthase, mitochondrial Kit ELISA, atp-citrate synthase Kit ELISA, ATP citrate lyase a Kit ELISA, ACLY Kit ELISA, acly.S Kit ELISA, LOC587157 Kit ELISA, acly Kit ELISA, ATPCL Kit ELISA, LOC5564509 Kit ELISA, CpipJ_CPIJ010291 Kit ELISA, Bm1_26245 Kit ELISA, ACL Kit ELISA, Acly Kit ELISA, aclya Kit ELISA

UniProt: P53396

Pathways: L'effet Warburg