KLKB1 Kit ELISA

N° du produit ABIN6730355

Détail du produit KLKB1 Kit ELISA

Antigène: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse KLKB1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Mouse KLKB1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: KLKB1 (Kallikrein B, Plasma (Fletcher Factor) 1 (KLKB1))

Autre désignation: KLKB1 (KLKB1 Produits)

Synonymes: KLK3 Kit ELISA, PPK Kit ELISA, APS Kit ELISA, Kal-3 Kit ELISA, Kal3 Kit ELISA, Klk3 Kit ELISA, PSA Kit ELISA, KALP15 Kit ELISA, Kalp1 Kit ELISA, KLKB1 Kit ELISA, DKFZp459J2015 Kit ELISA, klkb1 Kit ELISA, kallikrein B1 Kit ELISA, kallikrein B, plasma 1 Kit ELISA, plasma kallikrein Kit ELISA, Plasma kallikrein Kit ELISA, kallikrein B, plasma (Fletcher factor) 1 Kit ELISA, coagulation factor XI Kit ELISA, kallikrein B1 L homeolog Kit ELISA, KLKB1 Kit ELISA, Klkb1 Kit ELISA, CpipJ_CPIJ004042 Kit ELISA, CpipJ_CPIJ014955 Kit ELISA, CpipJ_CPIJ020108 Kit ELISA, VAA_02266 Kit ELISA, LOC100549617 Kit ELISA, klkb1 Kit ELISA, klkb1.L Kit ELISA

UniProt: P26262

Pathways: Système du Complément, Brown Fat Cell Differentiation