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DDR2 Kit ELISA

DDR2 Reactivité: Souris Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN6730477
  • Antigène Voir toutes DDR2 Kits ELISA
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Reactivité
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Mouse DDR2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Mouse DDR2.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 µl to 1 µl volumes
    • Adjustable 1-25 µl pipettes for reagent preparation
    • 100 µl and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product DDR2 Kit ELISA
  • Indications d'application
    Optimal working dilution should be determined by the investigator.
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 µl of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4°C.
    4. Add 100 µl of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 µl of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 µl of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes DDR2 Kits ELISA
    DDR2 (Discoidin Domain Receptor tyrosine Kinase 2 (DDR2))
    Autre désignation
    DDR2 (DDR2 Produits)
    Synonymes
    MIG20a Kit ELISA, NTRKR3 Kit ELISA, TKT Kit ELISA, TYRO10 Kit ELISA, AW495251 Kit ELISA, Ntrkr3 Kit ELISA, tyro10 Kit ELISA, DDR2 Kit ELISA, Tyro10 Kit ELISA, ddr2 Kit ELISA, si:ch211-193c2.1 Kit ELISA, discoidin domain receptor tyrosine kinase 2 Kit ELISA, discoidin domain receptor family, member 2 Kit ELISA, discoidin domain receptor family, member 2, like Kit ELISA, DDR2 Kit ELISA, Ddr2 Kit ELISA, ddr2l Kit ELISA
    UniProt
    Q62371
    Pathways
    Signalisation RTK
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